Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: FUR inhibition on colony formation in lung cancer cells. (a) Cells were treated with various concentrations of FUR for 24 h, after which morphological changes were observed using an AxioCam HRC CCD phase contrast microscope. Bar: 20 μ m. (b) Cells were treated with various concentrations of FUR for 24 h. After treatment, cells were suspended and reseeded into 6-well plates at a density of 200 cells per well (A549) or 1000 cells per well (95-D) and then fixed and stained with 4% PFA and crystal violet after one week. The representative images of colony formation assay were obtained. Bar: 2 mm.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques: Inhibition, Microscopy, Staining, Colony Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: FUR induces ER stress in lung cancer cells. (a) Effect of FUR on the mRNA levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h, and mRNA levels were determined by RT-PCR. (b) Effect of FUR on the protein levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h or 24 h, and protein levels were determined by Western blot. (c) Immunocytochemical staining was conducted to detect the expression of CHOP in nuclei. Cells were treated with 80 μ M FUR for 12 h and the cells were fixed and stained with anti-CHOP antibody (green) and Hoechst 33342 (blue). Bar: 10 μ m.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: CI values of FUR at concentrations applied in combination with DOX or TAX in different lung cancer cell lines.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques:

Journal: PLoS ONE

Article Title: Molecular Typing of Lung Adenocarcinoma on Cytological Samples Using a Multigene Next Generation Sequencing Panel

doi: 10.1371/journal.pone.0080478

Figure Lengend Snippet: Mutations found in 24 lung adenocarcinoma cytology specimens sequenced for 22 cancer-related genes using the Ion AmpliSeq Colon and Lung Cancer Panel v1.

Article Snippet: Ten ng of each dilution point were subjected to Ion AmpliSeq Colon and Lung Cancer Panel analysis v1 (Life Technologies).

Techniques: